You need to first create a S3 bucket in AWS and then upload your ova file to that bucket.
To upload the OVA file using command-line method
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Download and install the AWS Command Line Interface.
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Use the aws s3 mb command to create a new bucket. Bucket names must be unique and should be DNS compliant:
aws s3 mb s3://my-bucket --region my-region
where, my-bucket is the name that you provide for your bucket and my-region is the region that you provide.
If you do not use the region option of the command, the bucket is created in the region specified in your configuration file.
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Upload the OVA file by running the following command:
aws s3 cp my-ova_file s3://my-bucket/my-ova-key
Where, my-ova_file is the path and name of your local ova file, my-bucket is the name of your bucket on S3 storage and my-ova-key is the key or alias name for the ova file in your bucket.
See Deploying the virtual appliances in AWS